| Character | Actor/ Actress |
| Serena/ Sailor Moon |
Tracey Moore
(eps. 1-11, 15, 21, 41)
Terri Hawkes (all others for seasons 1 and 2) |
| Amy Anderson/ Sailor Mercury | Karen Bernstein |
| Raye Hino/ Sailor Mars | Katie Griffin |
| Lita/ Sailor Jupiter | Susan Roman |
| Darien/ Tuxedo Mask |
Rino Romano
(1-11)
Toby Proctor (12-66) Vince Carraza (all others) |
| Mina/ Sailor Venus | Stephanie Morganstern |
| Luna | Jill Frappier |
| Artemis | Ron Rubin |
| Sailor Pluto/ Luna Ball |
Jill Frappier
(58)
Sabrina Grdevich (all others) |
| Molly Baker | Mary Long |
| Melvin | Roland Parliament |
| Andrew | Colin O'Meara |
| Rini/ Sailor Mini Moon |
Traci Hoyt
Stephanie Beard (S season) |
| Hotaru Tomoe/ Sailor Saturn | Susan Aceron |
| Queen Beryl | Naz Edwards |
| Jedite [sic] | Tony Daniels |
| Neflite [sic] | Kevin Lund |
| Zoycite [sic] | Kirsten Bishop |
| Malachite | Dennis Akayama |
| Empress Metallia/ Negaforce | Maria Vacratsis |
| Alan | Vince Carraza |
| Anne | Sabrina Grdevich |
| Doom Tree/ Tree of Life | Liz Hannah |
| Catzy |
Alice Poon
Mary Long |
| Avery | Jennifer Griffiths |
| Prizma | Norma Dell'Agnese |
| Rubius | Rob Tinkler |
| Wiseman | Tony Daniels |
| Queen Serenity | Wendy Lyon |
| Sammy | Julie Lemieux |
| Serena's Mom | Barbara Radecki |
| Serena's Dad | David Hubard |
| Patricia Haruna | Nadine Rabinovitch |
| Grandpa | David Fraser |
| Chad | Steve Bednarski |
| Announcer | Chris Wiggins |
| Monster of the Day |
Harvey Atkins
Lindsay Collins Lisa Dalbello Tony Daniels David Fraser Terri Hawkes Elva Mai Hoover Loretta Jafelice Julie Lemieux Allison Sealy-Smith Maria Vacratisis |
| Background voices |
Steve Bednarski
Chris Britton Lindsay Collins Tony Daniels David Fraser Hillary Goldhar Loretta Jafelice Julie Lemieux Roland Parliament Alice Poon Nadine Rabinovitch Greg Swanson |
| Mr. Baxter | Chris Wiggins |
| Game Machine Joe | Rino Romano |
| Jordan (baby) | Tony Daniels |
| Peter Fisher | Joel Feeney |
| Greg | Eric Kimmel |
| Peggy Jones | Katherine Trowell |
| Misha | Jeff Lumby |
| Jenelle | Tracey Hoyt |
| Mika | Kathy Laskey |
| Mika's Mother | Wendy Lyon |
| Chess Tower owner | Roland Parliament |
| Countess Rose | Wendy Lyon |